Pulsatilla koreana Nakai Downregulates C/EBPs/PPARγand Suppresses Fatty Acid Synthase via activation of AMPKα in 3T3-L1 cells
By: Kim, N. H
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Contributor(s): Kim, H. J.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2019Edition: Vol.81(2), Mar-Apr.Description: 219-225p.Subject(s): PHARMACEUTICSOnline resources: Click here
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Indian journal of pharmaceutical sciencesSummary: Pulsatilla koreana Nakai, or pasque flower has been used as a medicinal plant for the treatment of amoebic dysentery, malaria, and internal haemorrhoids. The extract of Pulsatilla koreana Nakai, itself, has not been yet investigated for potential antiobesity effects. In this study, the antiobesity effect was evaluated on preadipocyte differentiation of Pulsatilla koreana Nakai extracted by 70 % ethanol in 3T3-L1 cells. Pulsatilla koreana Nakai ethanol extract at doses of 20 μg/ml significantly decreased lipid droplet accumulation, the expression of transcription factors, including CCAAT/enhancer-binding protein α, β, δ, peroxisome proliferator-activated receptor gamma and mRNA levels of fatty acid synthase. Moreover, Pulsatilla koreanaNakai ethanol extract also activated the phosphorylation of 5' adenosine monophosphate-activated protein kinase α. In conclusion, Pulsatilla koreana Nakai ethanol extract suppressed preadipocyte differentiation via down-regulation of CCAAT/enhancer-binding proteins, peroxisome proliferator-activated receptor gamma, and fatty acid synthase by phosphorylation of 5'-adenosine monophosphate-activated protein kinase α in adipocytes. This data suggested that Pulsatilla koreana Nakai ethanol extract be further explored as a new preventive and therapeutic agent against obesity.
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School of Pharmacy Archieval Section | Not for loan | 2021019 |
Pulsatilla koreana Nakai, or pasque flower has been used as a medicinal plant for the treatment of amoebic dysentery, malaria, and internal haemorrhoids. The extract of Pulsatilla koreana Nakai, itself, has not been yet investigated for potential antiobesity effects. In this study, the antiobesity effect was evaluated on preadipocyte differentiation of Pulsatilla koreana Nakai extracted by 70 % ethanol in 3T3-L1 cells. Pulsatilla koreana Nakai ethanol extract at doses of 20 μg/ml significantly decreased lipid droplet accumulation, the expression of transcription factors, including CCAAT/enhancer-binding protein α, β, δ, peroxisome proliferator-activated receptor gamma and mRNA levels of fatty acid synthase. Moreover, Pulsatilla koreanaNakai ethanol extract also activated the phosphorylation of 5' adenosine monophosphate-activated protein kinase α. In conclusion, Pulsatilla koreana Nakai ethanol extract suppressed preadipocyte differentiation via down-regulation of CCAAT/enhancer-binding proteins, peroxisome proliferator-activated receptor gamma, and fatty acid synthase by phosphorylation of 5'-adenosine monophosphate-activated protein kinase α in adipocytes. This data suggested that Pulsatilla koreana Nakai ethanol extract be further explored as a new preventive and therapeutic agent against obesity.
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